New motile intranuclear symbionts of ciliate

Vishniakov A., Skoblo I., Rodionova G.

Biological Research Institute of S-Petersburg State University, Saint-Petersburg, Russia

First motile form of intranuclear symbiont bacteria of ciliates were found in Paramecium multimicronucleatum, collected in two very remote areas: USA and Moldova (former USSR). The symbionts inhabit only in a macronucleus of ciliates. Their principal peculiarity is an ability to move inside all volume of the macronucleus. The velocity of locomotion is about 10-15 mkm/s. The length of bacteria varies from 3-4 mkm, and width is about 1 mkm. Cell form is different: oval, or long rod-shaped. Bacteria have numerous flagella, which arrange on all perimeter of the symbiont cell from Moldova and, apparently, have a local place of attachment on the cell surface of symbionts from USA. Length of flagella is equal to several lengths of cell, and a diameter of one is about 20 nm in both forms of symbionts. Flagella have not any piles or other appendages. The symbionts have a nucleoid arranged in a centre of cell . In situ hybridization with Holospora-specific probe (most studied genus of ciliate intranuclear symbyonts) did not show any positive signal in both forms. Fine structure of these new symbionts was investigated.

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