Heterologous membrane proteins in Saccharomyces cerevisiae: expression and targeting
Lang, C., Terng, H.-J., Polakowski, T., Prinz, B., Stahl, U.
Technische Universität Berlin, Institut für Biotechnologie, FG Mikrobiologie und Genetik, Gustav-Meyer-Allee 25, D-13355 Berlin, Germany
The yeast Saccharomyces cerevisiae is widely used as a host for the synthesis and analysis of heterologous proteins. It has also proven useful to express faithfully membrane proteins of mammalian and bacterial origin. Functional expression of theses proteins often requires their correct localisation in the cell. This targeting process which is mediated by intramolcular signal peptides is, however, not always predictable and may lead to locations other than in the homologous cell system. We have analysed the expression of the halobacterial protein bacterio-opsin, the model protein for seven- transmembrane proteins, and the human transferrin receptor, a plasma membrane located type II membrane protein (1,2). While the human transferrin receptor is expressed and correctly targeted to the plasma membrane, the heptahelical protein bacterioopsin is predominantly located in the vacuolar membrane when expressed in the yeast Saccharomyces cerevisiae. We here report on this subcellular localisation and targeting of these membrane proteins in yeast and on strategies to influence the targeting process by altering the targeting signals.
(1) Lang-Hinrichs C., I. Queck, G. Büldt, U. Stahl, V. Hildebrandt (1994) The archaebacterial
membrane protein bacterio-opsin is expressed and N- terminally processed in the yeast Saccharomyces cerevisiae. Mol. Gen. Genet. 244: 183 - 188
(2) Terng H.J., R. Geßner, H. Fuchs, U. Stahl, C. Lang (1997) Human transferrin receptor is active and plasma membrane-targeted in yeast. FEMS Microbiol. Letters (im Druck)
| LOCATION |
DATE |
TIME |
| Lecture Hall I |
Monday, April 6 |
06:10 pm |