The effect of high-light fluxes and low temperature acclimation on the expression of a nuclear encoded thylakoid membrane protein
Petzold, B.1, Montané, M.-H.2, Kloppstech, K.1
1Institut für Botanik, Universität Hannover, Herrenhäuser Straße 2, D-30419 Hannover, Germany,
2CEA, Centre de Cadarache, Département d’Ecophysiologie Végétale et de Microbiologie, F-13108 St. Paul-lez-Durance cedex, France
We investigated the effect of adaptation of barley to a combination of high light and cold on the photosynthetic and light harvesting properties. It was found that high light but preferentially the combination of both stresses favoured the trimerization of LHC complexes but had comparatively small effect on the protein composition of the thylakoid membrane. The same conditions caused a 100-fold accumulation of ELIPs. Using electrophoresis in the presence of decylmaltoside we could demonstrate for the first time that ELIPs are contained in higher molecular mass complexes of up to 500 kDa, but the composition of the complexes could not be determined. The xanthophyll content increases linearly with cellular development, the highest amount is observed in the light-exposed apical part of the leaf where light stress is at its highest. High light mainly induced the accumulation of antheraxanthin along the light-stressed leaf gradient and that of zeaxanthin only in the very tip of the leaf. Even though ELIP and antheraxanthin accumulate with the same pattern in the light and within the leaf gradient, we cannot derive a strict correlation. The latter observation makes it difficult to conclude that ELIPs may function as carotenoid binding proteins.
| LOCATION |
DATE |
TIME |
| Lecture Hall I |
Wednesday, April 8 |
02:20 pm |