Cellulose acetate electrophoresis of isozymes of the beech mycorrhiza Fagus sylvatica – Xerocomus chrysenteron
Fiedler O. and Rothe G.M.
Institut für Allgemeine Botanik, FB Biologie, Johannes Gutenberg-Universität, Mainz, Germany
European beech (Fagus sylvatica L.) contributes to a large extent to the biodi-versity of soil organisms because it forms, with its root tips, a symbiotic entity with many different soil fungi. Whereas the intra- and interpopulational variability of Euro-pean beech has been studied severalfold, genetic studies on its symbiotic root fungi are not available. Under certain requirements multiple enzyme systems (''isozymes'') can be used for such studies. The frequently occurring symbiotic entity (mycorrhiza) between Fagus sylvatica and the basidiomycetal fungus Xerocomus chrysenteron was sampled at two beech stands north of Wiesbaden at the State of Hessen. Mycorrhizal parts of root clusters with this fungus and non-mycorrhizal parts of the very same cluster were investigated for the occurrence of isozymes using Cellulose Acetate electrophoresis and histochemical enzyme staining. Of 21 enzyme systems studied 14 could be visualized. The enzyme systems glucose-phosphate isomerase, glutamate dehydrogenase (NAD-dependent) and peroxidase were active only in beech roots. Arabitol dehydrogenase, catalase and trehalase were specific for X. chrysenteron but showed no variability (two populations, 38 samples). Esterase, leucine aminopeptidase, mannitol dehydrogenase, peptidase and acid phosphatase were also of fungal origin but polymorphic. Aspartate aminotransferase, glucose-6-phosphate dehydrogenase, malate dehydrogenase and malate enzyme were expressed both in root tissue and fungal hyphae; of these glucose-6-phosphate dehydrogenase and malate dehydrogenase from X. chrysenteron were polymorph. The variability of allelic enzymes at the loci EST-A, EST-B of esterase, G6PDH-B of glucose-6-phosphate dehydrogenase, LAP-A, LAP-B of leucine aminopeptidase, MDH-C of malate dehydrogenase, MND-A of mannitol dehydrogenase, PEP-A, PEP-B of peptidase and ACP-A, ACP-B of acid phosphatase can be used for populational genetic studies of the ectomycorrhizal symbiosis of F. sylvatica and X. chrysenteron.
| LOCATION |
DATE |
TIME |
| Lecture Hall II |
Thursday, April 9 |
05:30 pm |