The first complete DNA sequencing of Ti plasmid suggests the relationship
between T-DNA transfer and trans-kingdom conjugation ?
Yoshida, K., Suzuki, K., Ohta, N., Hattori, Y., Uraji, M., Katoh, A., Iwata, K.,
Murata, K.
Department of Biological Science, Faculty of Science, Hiroshima University,
Higashi-Hiroshima 739-8526, Japan
By trans-kingdom conjugation and agrobacterial infection, DNA can move
directly from prokaryotic bacteria into eukaryotic cells. In general, the
former is a whole plasmid genome transfer while the latter is a small limited
T-DNA transfer of agrobacterial infectious Ti or Ri plasmids (see ref. (1)).
The Ti and Ri themselves are also typical bacterial conjugative plasmids. In
order to know the relationship between infectious T-DNA transfer and trans-
kingdom conjugation, we have isolated a wide-host range nopaline type Ti
plasmid (named pTi-SAKURA) in Agrobacterium tumefaciens MAFF301001 which
is infectious to a Japanese cherry tree, Sakura, and subsequently sequenced
its whole genome of 203 kbp. The complete DNA sequencing revealed pTi-SAKURA's
unique characteristics. Besides common Ti genes of vir, T-DNA, rep, tra,
trb and ori-T, it has several unusual genes including nodQ,
etherase and sucrose phosphorylase genes which probably contribute to the
expansion of its host range specificity, and more than 20 open reading frames.
In this paper, we describe about the genome structure of pTi-SAKURA and the
relationship between T-DNA transfer and trans-kingdom conjugation on the basis
of complete genome sequencing.
(1) Yoshida, K. et al.: Trans-kingdom conjugation as a
model gene transfer from endosymbionts to nucleus during the organelles origin.
In intertaxonic Combination and Symbiotic Adaptation (Endocytobiology VI).
pp.153-163, Springer-Verlag (1997).
| LOCATION |
DATE |
TIME |
| Lecture Hall II |
Thursday, April 9 |
04:30 pm |