"From Symbiosis to Eukaryotism" ENDOCYTOBIOLOGY VII Proceedings of the International Congress on Endocytobiology, Symbiosis and Biomedicine Freiburg im Breisgau, Germany April 5 - 9, 1998 Editors: Edgar Wagner, Johannes Normann, Hubert Greppin, Johannes H.P. Hackstein, Reinhold G. Herrmann, Klaus V. Kowallik, Hainfried E.A. Schenk, Joseph Seckbach University of Freiburg, University of Geneva, 1999 Vol. I + II, 800 pages ISBN 2-88164-010-9, ISBN 2-88164-011-7, ISBN 2-88164-012-5

Contents

• Introductory Remarks

• How scientific is science? A simple question without a simple answer

  Jerome F. Fredrick Memorial Symposium

• The origins of the protists: Glycogen storage

• Diversification of early life and the origin of the three domains: A proposal

• Mitochondrial origin by sulfur symbiosis

• Non-symbiotic eukaryogenesis - Membrane evolution theory

• Origin of eukaryotic cells by intra-cellular natural selection

• From genesine to glycogen

• Ubiquity of life as related to certain extremophiles: Mini review on Cyanidia

• The origin and evolution of the eukaryotic cell - The nucleus as endosymbiont

• The possible origin of the bacterial flagellum

• Phytogene sequence: From cyanobacteria to plastids

  Session A. Symbioses (Phenomenology and Phylogeny)

• Establishment and maintenance of diatom endosymbiosis in the larger foraminifer, Amphistegina lobifera

• The ciliate Euplotidium itoi and its episymbionts (epixenosomes) influence each other

• Genetic consequences of the intranuclear symbiosis Paramecium-Holospora analyzed by PFGE

• Motile intranuclear symbionts of ciliate Paramecium multimicronucleatum

• Characteristics of association between ruminant and rumen ciliates

• Leafhopper endosymbiosis in embryological perspective - A survey of evolutionary and functional implications

• Wolbachia can rescue from cytoplasmic incompatibility while being unable to induce it

• Symbiotic association between the acoel Convolutriloba longifissura and the alga Tetraselmis sp.

• Infection of cortical cells in non-nitrogen-fixing nodules of Alnus glutinosa

• Cellulose acetate electrophoresis of the beech ectomycorrhiza Fagus sylvatica - Xerocomus chrysenteron

  Session B. Pro- and Eukaryotic Cells (Endocytobiological / Endocytotechnical Aspects)

• Protein phylogenies and a new proposal for the classification of organisms unifying cell structure and molecular sequence data

• Tentative answers to four arbitrary questions about organelle genome reduction

• Chloroplast genomics: A new approach to study plastid genome evolution

• Lessons from nucleomorphs and chloroplasts

• How have apicomplexan plastids evolved? A hypothesis

• Identification of Mg-protoporphyrin IX as a chloroplast signal that mediates the expression of nuclear genes

• Mitochondrial DNA in Pleurotus (fungi, basidiomycetes): Structure, inheritance and correlation with nuclear background

• Interaction between cytosolic and nuclear calcium levels in relation to calmodulin gene expression in Nicotiana plumbaginifolia

• Temporal patterns of land plant evolution suggest extensive polyphyly

• A perforated armour: The dilemma of the muroplasts (cyanelles) of the glaucocystophyceae

• Heterologous membrane proteins in Saccharomyces cerevisiae: Intracellular targeting of bacterio-opsin

• Stabilization and translation of the psbD mRNA in Chlamydomonas reinhardtii

• Mobile introns in yeast mitochondria

• Reverse genetics in higher plant plastids

• Two proteins of chloroplasts are immunologically related to the bacterial protease La

• Analyzing "sticky" proteins - Quantitation of membrane binding of human mitochondrial creatine kinase using surface plasmon resonance with immobilized lipid vesicles

  Session C. Biomedical Aspects of Cancer (Endocytobiological View)

• Pleomorphism of "bacterioplasts" and mitochondria as an indicator of carcinogenesis

• Effect of granulocyte colony-stimulating factor on cell proliferation, p53 and VEGF expression of the ovarian cancer cell line HEY

• Are those 13 proteins really unimportable?

• Nitric oxide in mitochondria: Formation and consequences

Session D. Theoretical / Applied Aspects of Symbiosis / Endocytobiology / Medicine

• Interrelationship of cytoarchitecture and glucose metabolism

• Reciprocal modulation of actin polymerisation, cell motility and glycolytic enzyme activity

• RNA replication in evolutive biotechnology

• Selection of RNA aptamers that bind to a peptide of the canyon region of Human Rhinovirus 14

• Generation of biased peptide libraries based on evolutionary constraints

• Visualizing Sequence Space by Self-Organizing Feature Maps: Classification of Protein Targeting Signals

• Arabidopsis thaliana floral stem growth: Possible intervention of two different rhythms

• Signal-induced cytosolic Ca²⁺ oscillations, quantal Ca²⁺ release and inositol 1,4,5-trisphosphate metabolism: A unified theoretical approach

• External signals and internal oscillation dynamics: Coding and amplification of coherent and noisy signals

• Experimental evidence for an active cellular oscillator interfering with external fields

• Jakob von Uexküll's biosemiotics and the biochemical model

• Thure von Uexküll: Symbiosis of biology, medicine, and semiotics

• Symbiosis is semiosis

• The state of endosymbiotic theory in Eastern Europe

• "Holistic Darwinism" and the Synergism Hypothesis

• Paul Alfred Weiss (1898-1989): The factory of the cell and molecular ecology

• Planetary superorganism and symbiogenesis

• Close microbial symbioses and systematics

• The relationship between optical density, metabolic and budding activity investigated in yeast cells using the technique of laser phase microscopy compared with fluorescence microscopy, scanning electron microscopy, and scanning near-field optical microscopy

• The Robot-Microbeam technology for single cell micropreparation and non-contact laser microinjection

• Further to the evolution of eukaryotism

• Index of contributors